Mediastinal lymph node involvement is the most important factor which determines the treatment and prognosis in NSCLC. In this study, the role of EBUS-TBNA was examined in evaluating intrathoracic lymph node in patients diagnosed with preoperative NSCLC.EBUS-TBNA was performed on fourty-four male cases (average age 59.8) diagnosed with preoperative NSCLC between March 2010-January 2012. Thorax-CT and PET-CT were performed on all patients before staging. Hystopathological evaluation results of lymph nodes which were dissected during the surgery were compared with citopathological evaluation results of the lymph nodes which were taken through EBUS-TBNA.Sixteenright paratracheal (36,4%), 23 left paratracheal (52,3%) and 40 subcarinal (90,9%) lymph nodes were sampled through EBUS-TBNA. On the other hand, paratracheal lymph nodes were removed from 41 cases (93,2%) and subcarinal lymph nodes were removed from 43 cases (97,7%) through surgical procedure. Ultrasonograhic sizes of the right paratracheal lymph nodes which were sampled through EBUS-TBNA was 8,88mm, left paratracheal lymph nodes was 9,23mm and subcarinal lymph nodes was 11,66mm. As a result of the statistical analysis, sensitivity, specifity, PPV, NPV and accuracy of the EBUS-TBNA in NSCLC were identified as 66.7%, 100%, 100%, 97.6% and 97.7% respectively. Thus, in addition to clinician experience, tomogrophic, bronchoscopic and ultrasonograhic correlation of the lymph node which will be sampled and sufficient number of aspiration, EBUS-TBNA can be preferred as an alternative to invasive techniques due to its high diagnostic accuracy and accurate staging in NSCLC patients.